| Alachua to Olympia Heights (Florida) | 309.25 mi (497.69 km) |
| Alachua to Omaha (Nebraska) | 1,098.24 mi (1,767.45 km) |
| Alachua to On Top of the World Designated Place (Florida) | 47.92 mi (77.12 km) |
| Alachua to Onalaska (Wisconsin) | 1,086.25 mi (1,748.15 km) |
| Alachua to Oneida (New York) | 993.15 mi (1,598.33 km) |
| Alachua to Oneonta (New York) | 967.82 mi (1,557.55 km) |
| Alachua to Ontario (California) | 2,070.82 mi (3,332.67 km) |
| Alachua to Ontario (Oregon) | 2,123.71 mi (3,417.78 km) |
| Alachua to Opa-locka (Florida) | 300.53 mi (483.66 km) |
| Alachua to Opelika (Alabama) | 262.50 mi (422.46 km) |
| Alachua to Opelousas (Louisiana) | 575.60 mi (926.34 km) |
| Alachua to Orange (California) | 2,083.70 mi (3,353.40 km) |
| Alachua to Orange (Texas) | 675.29 mi (1,086.78 km) |
| Alachua to Orange City (Florida) | 92.59 mi (149.01 km) |
| Alachua to Orangeburg (South Carolina) | 273.70 mi (440.48 km) |
